Scientific Data

Western Blot

Detection of Human TSPAN8 antibody by Western Blot.

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Detection of Human TSPAN8 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human TSPAN8 Monoclonal Antibody (Catalog # MAB4734) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for TSPAN8 at approximately 35 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

Detection of TSPAN8 antibody in HT‑29 Human Cell Line antibody by Flow Cytometry.

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Detection of TSPAN8 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Rat Anti-Human TSPAN8 Monoclonal Antibody (Catalog # MAB4734, filled histogram) or isotype control antibody (MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry

TSPAN8 antibody in HT-29 Human Cell Line by Immunocytochemistry (ICC).

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TSPAN8 in HT‑29 Human Cell Line. TSPAN8 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Rat Anti-Human TSPAN8 Monoclonal Antibody (Catalog # MAB4734) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry

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Detection of TSPAN8 in HT‑29 Human Colon Adenocarcinoma Cell Line (Positive) and Daudi Human Burkitt's Lymphoma Cell Line (Negative) Cells. TSPAN8 was detected in immersion fixed HT‑29 Human Colon Adenocarcinoma Cell Line (Positive) and Daudi Human Burkitt's Lymphoma Cell Line (Negative) Cells using Rat Anti-Human TSPAN8 Monoclonal Antibody (Catalog # MAB4734) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Human TSPAN8 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TSPAN8 in Western blots.

Source

Monoclonal Rat IgG_2B Clone # 458811

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human TSPAN8
Met1-Lys237 (Ile35Val)
Accession # AAH05246

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

3-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

hTSPAN8 MAb (Cl 4588 (25 ug)