Purified Mouse Anti-β-Tubulin(5H1)

Unconjugated

β-Tubulin

Mouse IgM, κ

Tubulin is a highly conserved protein with a molecular weight of ~50 kD.  The self-assembly of tubulin leads to microtubules, hollow cylinders that are one of the major components of the eukaryotic cytoskeleton.  Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.  There are two main classes of tubulin isoforms, α- and β-tubulin, which are usually products of separate genes.  Microtubules are made from protofilaments, strings of alternating α- and β-tubulin spaced 4 nm apart and pointing in the same direction.  Tubulin can be posttranslationally modified in several ways, including phosphorylation, acetylation, glutamylation, and detyrosination.  For example, microtubules that turn over slowly tend to be acetylated and detyrosinated. The 5H1 monoclonal antibody reacts with β-tubulin.  It does not cross-react with α-tubulin.

Tubulin is a highly conserved protein with a molecular weight of ~50 kD.  The self-assembly of tubulin leads to microtubules, hollow cylinders that are one of the major components of the eukaryotic cytoskeleton.  Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.  There are two main classes of tubulin isoforms, α- and β-tubulin, which are usually products of separate genes.  Microtubules are made from protofilaments, strings of alternating α- and β-tubulin spaced 4 nm apart and pointing in the same direction.  Tubulin can be posttranslationally modified in several ways, including phosphorylation, acetylation, glutamylation, and detyrosination.  For example, microtubules that turn over slowly tend to be acetylated and detyrosinated. The 5H1 monoclonal antibody reacts with β-tubulin.  It does not cross-react with α-tubulin.

Mouse IgM, κ

5H1

0.5 mg/ml

Western blot (Routinely Tested) Bioimaging, Immunohistochemistry (Tested During Development)

Human (QC Testing), Mouse, Rat, Bovine (Tested in Development)

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(5) 1. Brown KD, Wood KW, Cleveland DW. The kinesin-like protein CENP-E is kinetochore-associated throughout poleward chromosome segregation during anaphase-A. J Cell Sci. 1996; 109:961-969. (Biology). 2. Cho J-H, Johnson GVW. Primed phosphorylation of tau at Thr231 by glycogen synthase kinase 3β (GSK3β) plays a critical role in regulating tau's ability to bind and stabilize microtubules. J Neurochem. 2004; 88:349-358. (Biology). 3. Helfand BT, Mikami A, Vallee RB, Goldman RD. A requirement for cytoplasmic dynein and dynactin in intermediate filament network assembly and organization. J Cell Biol. 2002; 157(5):795-806. (Biology). 4. Prahlad V, Yoon M, Moir RD, Vale RD, Goldman RD. Rapid movements of vimentin on microtubule tracks: kinesin-dependent assembly of intermediate filament networks. J Cell Biol. 1998; 143(1):159-170. (Biology). 5. Wang Y, Loomis PA, Zinkoswski RP, Binder LI. A novel tau transcript in cultured human neuroblastoma cells expressing nuclear tau. J Cell Biol. 1993; 121(2):257-267. (Biology).