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Ubiquitinylation Kit


品牌: Enzo Life








来源宿主:
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实验应用:
Uses:
1. Ubiquitinylation of target proteins in presence of dedicated E3 ligase. Panel of E2s provided for generation of E2-Ub thioester conjugates for testing vs. specific E3/target combinations. For example: ubiquitinylation of p53 in the presence of mdm2 (E3) and UbcH5b (E2)10.
2. Activation of Ub for thioester conjugation to
Uses:
1. Ubiquitinylation of target proteins in presence of dedicated E3 ligase. Panel of E2s provided for generation of E2-Ub thioester conjugates for testing vs. specific E3/target combinations. For example: ubiquitinylation of p53 in the presence of mdm2 (E3) and UbcH5b (E2)10.
2. Activation of Ub for thioester conjugation to
产品介绍
产品信息
宿主
无

试剂盒组分
20X Ubiquitin Activating Enzyme Solution
Human recombinant E1 (His6-tagged), 125µl provided, Product No. BML-UW9410

组成成分
重组泛素激活酶E1蛋白、重组泛素结合酶E2蛋白、重组泛素蛋白、腺嘌呤核苷三磷酸溶液、磷酸盐缓冲液、盐酸盐缓冲液

应用
实验应用
Uses:
1. Ubiquitinylation of target proteins in presence of dedicated E3 ligase. Panel of E2s provided for generation of E2-Ub thioester conjugates for testing vs. specific E3/target combinations. For example: ubiquitinylation of p53 in the presence of mdm2 (E3) and UbcH5b (E2)10.
2. Activation of Ub for thioester conjugation to

制备和贮存
保存
All kit components should be stored at -80°C to ensure stability and activity.

保存方式
-80°C
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