Rabbit anti-Vimentin Monoclonal Antibody(029-20)

WB result of anti-Vimentin antibody Primary antibody : Anti-Vimentin antibody at 1/1000 dilution Lane 1 : Daudi whole cell lysate 20 µg Lane 2 : Hela whole cell lysate 20 µg Lane 3 : A549 whole cell lysate 20 µg Lane 4 : Jurkat whole cell lysate 20 µg Negative control: Daudi whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 54 kDa Observed MW: 56 kDa Exposure time: 0.1 seconds

WB result of anti-Vimentin antibody Primary antibody : Anti-Vimentin antibody at 1/1000 dilution Lane 1 : NIH/3T3 whole cell lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 54 kDa Observed MW: 56 kDa Exposure time: 0.1 seconds

IHC shows positive staining in paraffin-embedded stromal of human colon cancer. Anti-Vimentin antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded stromal of human breast cancer. Anti-Vimentin antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded stromal of mouse colon. Anti-Vimentin antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

ICC shows positive staining in HeLa cells. Anti-Vimentin antibody was used at 1/250 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution.The cells were fixed with 100% methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were countersained with DAPI.