
产品介绍
产品信息
宿主
鼠

克隆类别
单抗

Product Details
The 13F3 monoclonal antibody reacts with mouse V-domain Ig suppressor of T cell activation (VISTA) also known as PD-1H and B7-H5. VISTA is a 309 aa type I transmembrane glycoprotein and a member of the Ig superfamily. VISTA is expressed on naïve and activated T cells, NK cells, macrophages, dendritic cells, and neutrophils. VISTA functions as a negative immune-checkpoint protein that suppresses T cell cytokine production and proliferation. VISTA is overexpressed by tumor-infiltrating lymphocytes, such as myeloid cells and regulatory T cells. Blockade of VISTA with the 13F3 antibody results in delayed tumor growth in mouse models of melanoma.Specifications
Isotype | Armenian hamster IgG |
---|---|
Recommended Isotype Control(s) | InVivoPlus polyclonal Armenian hamster IgG |
Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
Immunogen | EL4 cells overexpressing mouse VISTA-RFP and then boosted with VISTA-Ig fusion protein |
Reported Applications | in vivo blocking of VISTA signaling in vitro blocking of VISTA signaling |
Formulation | PBS, pH 7.0 Contains no stabilizers or preservatives |
Endotoxin* | <1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay |
Aggregation* | <5% Determined by SEC |
Purity | >95% Determined by SDS-PAGE |
Sterility | 0.2 μM filtered |
Production | Purified from cell culture supernatant in an animal-free facility |
Purification | Protein A |
RRID | AB_2736990 |
Molecular Weight | 150 kDa |
Murine Pathogen Tests* | Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
组成成分
InVivoPlus抗鼠VISTA抗体(抗鼠T细胞活化抑制因子蛋白抗体),磷酸盐缓冲液

应用
反应种属
mouse

背景
研究领域
Cancer Biology, Immune Checkpoint Protein, Immunology
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