Wnt Signaling Antibody Sampler Kit

1/12

Wnt Signaling Antibody Sampler Kit

品牌： CST

下载产品说明书

用小程序，查商品更便捷

对比

咨询

参数

分子量：

n/a

产品介绍

产品信息

抗原名称

Wnt Signaling

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu256 of human Wnt3a, Glu176 of human Wnt5a, Met988 of human LRP6, Gly176 of human Dvl2, and Ala217 of human Naked2. The monoclonal antibody for Axin1 is produced by immunizing animals with recombinant human Axin1. The phospho-specific polyclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1490 of human LRP6. The total polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the carboxy terminus of human Dvl3 and Gln227 of human Naked1. Polyclonal antibodies are purified by Protein A and peptide affinity chromotagraphy.

简单描述

Antibody Sampler Kit for studying in the research area.

分子量

n/a

应用

目标/特异性

Specificity/Sensitivity

Wnt3a (C64F2) Rabbit mAb detects transfected Wnt3a protein in L cells and the conditioned medium from L/Wnt3a cells. Phospho-LRP6 (Ser1490) Antibody detects endogenous levels of LRP6 protein when phosphorylated at Ser1490. The remaining antibodies in the Wnt Signaling Sampler Kit detect endogenous levels of their respective total proteins.

背景

The Wnt family includes several secreted glycoproteins that play important roles in animal development (1). Aberrant activation of Wnt signaling pathways is involved in several types of cancers (2). Wnt members bind to the Frizzled family of proteins and activate several signaling pathways (3). The canonical Wnt/β-catenin pathway requires a coreceptor from proteins belonging to the low-density lipoprotein receptor (LDLR) family, such as LRP5 and LRP6 (4). Upon stimulation with Wnt, LRP6 is phosphorylated by kinases such as GSK-3 and CK1 and subsequently recruits Axin to the membrane (5-7). In the absence of Wnt stimulation, the multidomain scaffold proteins Axin1 and Axin2 negatively regulate Wnt signaling. Axin1 complexes with APC, GSK-3β, Dvl and β-catenin and promotes the GSK-3β-mediated phosphorylation and subsequent degradation of β-catenin (8-9). The Disheveled (Dsh) proteins (Dvl1, Dvl2 and Dvl3) inhibit glycogen synthase kinase-3β, thereby promoting β-catenin stabilization (4,10). Naked inhibits the canonical Wnt/β-catenin pathway by binding to Dsh proteins and directing Dsh activity towards the planar cell polarity pathway (11-12). 1.Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305. 2.Giles, R.H. et al. (2003) Biochim Biophys Acta 1653, 1-24. 3.Kohn, A.D. and Moon, R.T. Cell Calcium 38, 439-46. 4.Logan, C.Y. and Nusse, R. (2004) Annu Rev Cell Dev Biol 20, 781-810. 5.Tamai, K. et al. (2000) Nature 407, 530-5. 6.Zeng, X. et al. (2005) Nature 438, 873-7. 7.Davidson, G. et al. (2005) Nature 438, 867-72. 8.Luo, W. and Lin, S.C. Neurosignals 13, 99-113. 9.Salahshor, S. and Woodgett, J.R. (2005) J Clin Pathol 58, 225-36. 10.Boutros, M. and Mlodzik, M. (1999) Mech Dev 83, 27-37. 11.Wharton, K.A. et al. (2001) Dev Biol 234, 93-106. 12.Yan, D. et al. (2001) Proc Natl Acad Sci U S A 98, 3802-7.

参考图片

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

Western blot analysis of extracts from NIH/3T3, C6, A549, BAEC and COS cells using Dvl3 Antibody.

Western blot analysis of total cell lysates and conditioned medium (CM) from L cells overexpressing mouse Wnt3a, compared with parental L cells, using Wnt3a (C64F2) Rabbit mAb #2721.使用Wnt3a (C64F2) 兔单抗#2721对过表达小鼠wnt3a的L细胞及对照组亲本L细胞的全细胞提取物和条件培养基进行western blot分析。

Western blot analysis of total cell lysates from HeLa, LN18, PANC1 cells and conditioned medium (CM) from HeLa cells, using Wnt5a/b (C27E8) Rabbit mAb #2530.使用Wnt5a/b (C27E8)兔单抗#2530对HeLa，LN18，PANC1全细胞提取物和HeLa细胞条件培养基提取物进行western blot分析。

Western blot analysis of total cell lysates from HeLa cells untreated, treated 5 hours with conditioned medium (CM) from L cells, or treated 5 hours with conditioned medium from L cells overexpressing Wnt3a, using Phospho-LRP6 (Ser1490) Antibody #2568.使用 Phospho-LRP6 (Ser1490)抗体#2568对未处理或用L细胞条件培养基或过表达Wnt3a的L细胞条件培养基处理5小时的HeLa细胞全细胞提取物进行进行western blot分析。

Western blot analysis of total cell lysates from various cell lines using Dvl2 (30D2) Rabbit mAb #3224.使用Dvl2 (30D2) 兔单抗#3224对多种细胞全细胞提取物进行western blot分析。

Western blot analysis of extracts from various cell lines using Dvl3 Antibody #3218.使用Dvl3抗体#3218对多种细胞提取物进行western blot分析。

Western blot analysis of total cell lysates from HepG2 and SW620 cells using Naked1 (C30F10) Rabbit mAb #2201.使用Naked1 (C30F10) 兔单抗#2201对HepG2和SW620细胞全细胞提取物进行western blot分析。

Western blot analysis of extracts from various cell lines using Naked2 (C67C4) Rabbit mAb #2073.使用Naked1 (C67C4) 兔单抗#2073对多种细胞提取物进行western blot分析。

Western blot analysis of extracts from various cell lines using Axin1 (C76H11) Rabbit mAb #2087.使用Axin1 (C76H11) 兔单抗#2087对多种细胞提取物进行western blot分析。

Western blot analysis of extracts from Hep G2 and HeLa cells using LRP6 (C47E12) Rabbit mAb.

Western blot analysis of extracts from Hep G2 and HeLa cells using LRP6 (C47E12) Rabbit mAb #3395.

Western blot analysis of total cell lysates from HeLa, LN18, PANC1 cells and HeLa cells conditioned medium (CM) using Wnt5a/b (C27E8) Rabbit mAb.

Western blot analysis of total cell lysates from various cell lines using Dvl2 (30D2) Rabbit mAb.

Western blot analysis of total cell lysates and conditioned medium from L cells overexpressing mouse Wnt3a, compared with parental L cells, using Wnt3a (C64F2) Rabbit mAb.

Western blot analysis of extracts from various cell lines using Axin1 (C76H11) Rabbit mAb.

Western blot analysis of total cell lysates from Rat2 and NIH/3T3 cells, untreated or treated with conditioned medium containing Wnt3a for 60 minutes and also treated with or without calf intestinal phosphatase (CIP), using Dvl2 (30D2) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Axin1 (C76H11) Rabbit mAb.

Western blot analysis of total cell lysates from HepG2 and SW620 cells using Naked1 (C30F10) Rabbit mAb.

Western blot analysis of extracts from various cell lines using Naked2 (C67C4) Rabbit mAb.

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

2915T

一键复制

询价

供应商现货

1Kit

选择数量

当前规格1件起购

配送至

预计5-6个工作日送达，快递: 免运费，若需干冰额外收费

询价大包装询价