XTT Cell Viability Kit

Figure 1. C2C12 cells were seeded at varying density in a 96-well plate and incubated overnight. The XTT assay solution was added to the plate and cells were incubated. The absorbance at 450 nm was measured at 1.0, 2.0, 3.0, 4.0, and 5.0 hours. C2C12细胞按照不同的密度种于96孔板中，孵育过夜。将XTT分析液加到板中然后孵育细胞，在450nm处，分别于1.0、2.0、3.0、4.0和5.0小时测吸光值。

Figure 2. C2C12 cells were seeded at 2x104 cells/well in a 96-well plate and incubated overnight. Cells were then treated with various concentrations of doxorubicin overnight. The cytotoxicity was measured using XTT Cell Viability Kit (red) and BrdU Cell Proliferation Assay Kit #6813 (blue) as shown in the left panel. The percentage inhibition in each assay was calculated and plotted in the right panel. Doxorubicin treatment can lead to cell DNA damage followed by cell cycle arrest. C2C12细胞按照2x10 4 cells/well 种于96孔板中孵育过夜。然后用不同浓度的链霉素处理细胞过夜。用XTT细胞活力分析试剂盒（红色）和BrdUp细胞增殖分析试剂盒（蓝色）检测细胞毒性，如左图所示。右图中显示在每个分析方法中的抑制比例。链霉素可以引起细胞DNA的损伤，进而使细胞周期停止。

Figure 3. HeLa cells were seeded at 1x104 cells/well in a 96-well plate and incubated overnight. Cells were then treated with various concentrations of staurosporine overnight. The cytotoxicity was measured using XTT Cell Viability Kit (red) and BrdU Cell Proliferation Assay Kit #6813 (blue) as shown in the left panel. The percentage inhibition in each assay was calculated and plotted in the right panel. Staurosporine is a nonspecific kinase inhibitor and induces cellular apoptosis. HeLa细胞按照1x10 4 cells/well种于96孔板中孵育过夜。然后用不同浓度的十字孢碱处理细胞过夜。用XTT细胞活力分析试剂盒（红色）和BrdUp细胞增殖分析试剂盒（蓝色）检测细胞毒性，如左图所示。右图中显示在每个分析方法中的抑制比例。十字孢碱是一种非特异性激酶抑制剂，可以引起细胞凋亡。